Step 1: Creating Transit Gateways
The journey begins with the deployment of Transit Gateways in the targeted regions. Attach relevant VPCs to each Transit Gateway to ensure they function correctly and facilitate smooth connectivity.

Step 2: Initiating Peering
Using the AWS Management Console, access the Transit Gateway service. This platform allows you to initiate a peering connection between the Transit Gateways located in various regions.

Step 3: Configuring Route Tables
Post peering, it's essential to update the route tables associated with each Transit Gateway. This update is critical for enabling the correct routing of traffic across the networked regions.

Step 4: Adjusting Security Measures
Modify Network Access Control Lists (ACLs) and Security Groups to permit traffic flow between the peered VPCs. This process involves setting up the right ports and protocols to ensure smooth communication between EC2 instances.

Step 5: Testing Connectivity
The final step is to verify connectivity. Employ tools like ping or use application-specific tests to confirm that EC2 instances in different regions can communicate without any hurdles.

Conclusion: Embracing Global Connectivity
Cross-region Transit Gateway peering in AWS is a game-changer for organizations looking to build a global, interconnected network infrastructure. This guide offers a clear roadmap for integrating resources across geographical boundaries, enabling direct communication between EC2 instances. Such a capability is invaluable for global enterprises, leading to scalable, resilient, and interconnected cloud architectures.